논문검색
Author Young Sook Lee, Moon June Cho, Jeung Hoon Lee, Woong Hee Lee, Jun Sang Kim
Place of duty Departments of 1Dermatology, and 2Radiation Oncology, College of Medicine, 3Department of Biology, College of Natural Sciences, 4Cancer Research Institute, Chungnam National University, Daejeon, Korea.
Title Detection of RTP801, a Gene That is Differentially Expressed in Irradiated HeLa Cells
Publicationinfo Cancer Research and Treatment 2004 Aug; 036(04): 263-270.
Key_word RTP801,Radiation,HeLa cells,Real time PCR
Full-Text
Abstract Purpose: To quantify the effects of irradiation on the expression levels of a differentially expressed gene, RTP801, in HeLa cells. Materials and Methods: Total RNA was isolated from irradiated and non-irradiated HeLa cells. A subtraction library was constructed, from which 88 random clones were screened. The expression patterns of one clone, detected by reverse Northern blotting, were quantified by real time RT-PCR, using CYBR green I dye. Results: RTP801, a hypoxia-inducible factor-I-responsive gene, was identified as a differentially expressed gene in HeLa cells exposed to X-ray. Real time RT-PCR showed that the mRNA levels of RTP801 were greatly diminished by radiation. Conclusions: These results suggest that down-regulation of hypoxia-inducible factor-I-responsive genes, such as RTP801, in irradiated HeLa cells may result in reductions in the radiotherapy resistance of tumor cells. (Cancer Research and Treatment 2004;36:263-270)